Barkley initially was listed as questionable to return, but the Giants quickly downgraded him to out.
He has an ankle injury, and a closeup of Barkley’s ankle on the Fox broadcast showed noticeable swelling.
Barkley limped off after an incompletion on a quick slant with 6:17 left in the first quarter. The team’s medical staff called for a cart on the sideline, and Barkley limped to it for the ride to the training room.
Barkley had two carries for 9 yards before leaving.
The Cowboys lead 3-0 after the first quarter.